GetORF - Finds and extracts open reading frames [Manual]

• Use Table Standard Standard(with alternative initiation codons) Vertebrate Mitochondrial Yeast Mitochondrial Mold, Protozoan, Coelenterate Mitochondrial and Mycoplasma/Spiroplasma Invertebrate Mitochondrial Ciliate Macronuclear and Dasycladacean Echinoderm Mitochondrial Euplotid Nuclear Bacterial Alternative Yeast Nuclear Ascidian Mitochondrial Flatworm Mitochondrial Blepharisma Macronuclear Chlorophycean Mitochondrial Trematode Mitochondrial Scenedesmus obliquus Thraustochytrium Mitochondrial
• Minimum nucleotide size of ORF to report(Any integer value)
• Find Translation of regions between STOP codons Translation of regions between START and STOP codons Nucleic sequences between STOP codons Nucleic sequences between START and STOP codons Nucleotides flanking START codons Nucleotides flanking initial STOP codons Nucleotides flanking ending STOP codons
• START codons at the beginning of protein products will usually code for Methionine
• Is the sequence circular?
• Set this to be false if you do not wish to find ORFs in the reverse complement of the sequence.
• set the number of nucleotides either side of that codon to output (find flanking sequence around a STOP or START codon)(Any integer value)