Plasmid Map 화면 구성

1. Seqloader : EnCyclon 에서 제공하는 시퀀스 입력 방법으로 보다 쉽게 시퀀스를 입력할 수 있습니다.

2. 시퀀스 입력창 : Map 을 그리기 위한 시퀀스를 입력하는 곳 입니다.

3. Plasmid Map 옵션

   • Analysis Options : Analysis Options을 체크하면 제한효소를 표시하는데 defalut Enzyme Set은 자주 사용하는 34개의 Enzyme으로 ApaI BamHI BglII EcoRI EcoRV HindIII HpaI KpnI MluI NcoI NdeI NheI NotI PstI PvuII SacI SalI SmaI SpeI XbaI XhoI ClaI PacI Nru1 Bsu36I PmeI XmaI SwaI AscI EagI BclI ScaI SexAI SfiI 입니다. Display Enzyme with position을 체크하면 제한효소를 표시할때 이름과 위치를 함께 표시해 줍니다. 나머지 Analysis Options은 RestrictionAnalyzer 과 동일합니다

   • Show Feature : Show Feature를 체크하면 시퀀스의 feature를 보여주는데 NGSF,Genbank 포멧일 경우 해당 포멧의 feature를 표시하고, 그 외의 포멧일경우 ORF search 결과값을 표시합니다.

   
   
   

4. 실행, 취소 : 시퀀스와 옵션 설정이 완료하면 실행하는 버튼과, 시퀀스 입력을 취소할 수 있는 클리어 버튼 입니다.

플라스미드맵 보기

1. Plasmid Map 페이지를 여십시오.

2. Genbank Accession : AB003468 시퀀스를 시퀀스 입력창에 입력합니다.

   시퀀스를 입력하는 방법은 여러 가지가 있습니다. 여기에서는 Seqloader를 이용하여 GENBANK에서 인터넷을 통해서 입력하겠습니다.

   1. Load Sequence 버튼을 클릭하여 Sequence Loader를 호출합니다.

   2. GENBANK Accession을 알고 있으므로, GENBANK Accession으로 시퀀스를 로드하기 위하여 콤보박스에서 GENBANK Accession을 선택하십시오.

   3. 옆의 텍스트 박스에 GENBANK Accession "AB003468"을 입력하십시오.

      

      
      
      

   4. 바로 옆의 "Load" 버튼을 클릭하십시오.

   5. 시퀀스가 시퀀스 입력박스에 들어갔을 것입니다.

      

      
      
      

   시퀀스를 입력하는 다른 방법은 EnCyclon 사용자 안내서의 "시퀀스 입력하는 방법"을 참조하십시오.

3. 기본 설정된 옵션으로 'GO' 버튼을 눌러 결과를 확인합니다.

   

   
   
   

Enzyme Site 표시

1. Plasmid Map 페이지를 여십시오.

2. Genbank Accession : AB003468 시퀀스를 시퀀스 입력창에 입력합니다.

3. Analysis Options (기본 옵션)을 체크합니다. 하위 메뉴로 Display Enzyme with position을 체크하면 제한효소를 표시할때 이름과 위치를 함께 표시해 줍니다. 나머지 Analysis Options은 RestrictionAnalyzer 과 동일합니다.

   

   
   
   

4. 'GO' 버튼을 눌러 결과를 확인합니다.

ORF Search

1. Plasmid Map 첫 페이지에서 Show Feature(기본 옵션)을 체크하면 시퀀스의 feature를 보여주는데 NGSF,Genbank 포멧일 경우 해당 포멧의 feature를 표시하고, 그 외의 포멧일경우 ORF search 결과값을 표시합니다.

2. 'GO' 버튼을 눌러 결과를 확인합니다.

3. 결과 화면에서 ORF Options 을 체크, 찾을 amino acid 길이를 '100' 이상으로 설정하여 'GO' 버튼을 누릅니다.

   

   
   
   

4. ORF Search 결과값은 맵에서 회색으로 표시되며, 시퀀스 Feature의 CDS 는 빨강색, Promoter 는 노랑색, Ohter 은 하늘색 으로 표시됩니다.

   

   
   
   

Feature 편집

1. 결과 화면에서 'GO' 버튼 아래의 feature 입력창에 아래와 같이 입력하고 'Add Feature' 버튼을 누릅니다.

   

   
   
   

2. 플라스미드맵과 아래의 테이블에 입력한 feature가 들어갔습니다.

   

   
   
   

3. 수정할 feature의 마지막 열의 'Edit' 버튼을 눌러 새창을 띄웁니다.

   

   
   
   

4. 원하는 항목의 값을 변경하고 'Edit Feature' 버튼을 누릅니다.

   

   
   
   

5. feature 가 변경되었습니다.

6. 테이블에서 삭제할 feature 의 처음 열에 있는 체크박스를 클릭하고 'DEL' 버튼을 누릅니다.

   

   
   
   

7. 선택한 feature 가 삭제 되었습니다.

   

   
   
   

저장 하기

1. 플라스미드맵 위에서 마우스 오른쪽 버튼을 클릭하여 '다른이름으로 그림저장' 을 선택하여 저장할 수 있습니다.

2. 'Save Sequence' 버튼을 눌러 feature 편집으로 변경된 시퀀스를 저장할 수 있습니다. 저장파일 형식은 'NGSF' 입니다.

pBacPAK8

1. pBacPAK8 시퀀스

   NG1.0 pBacPAK8 circular dsDNA 5538 bp DESCRIPTION Cloning vector pBacPAK8, complete sequence. FEATURE Source 1..5538 F Genbank:U02446 CDS 3683..4543 F Amp Promoter 1158..1250 F pPolyhedrin Other 1..1250 F AcMNPV Other 1327..2770 F AcMNPV Other 1252..1326 F MCS Other 1764 F poly_A Other 4950 C rep_origen SEQUENCE 1 aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag 61 aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa 121 caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt 181 accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt 241 ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca 301 cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa 361 accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg 421 caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt 481 gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt 541 gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag 601 ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct 661 tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat 721 ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt 781 ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt 841 tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg 901 ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt 961 acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta 1021 aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta 1081 aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc 1141 aatatatagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata 1201 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata cggatccctg 1261 caggcctcga gttcgaatct agaagatctg gtaccgagct cgaattcccg ggcggccgct 1321 taattaattg atccgggtta ttagtacatt tattaagcgc tagattctgt gcgttgttga 1381 tttacagaca attgttgtac gtattttaat aattcattaa atttataatc tttagggtgg 1441 tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt aaatattaaa 1501 tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg tttttccgaa 1561 ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc caaatcttgt 1621 agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa aggttcgacg 1681 tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt gtacaattga 1741 ctcgacgtaa acacgttaaa taaagcttgg acatatttaa catcgggcgt gttagcttta 1801 ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat 1861 tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc gatcaaattt 1921 gtagttgagc tttttggaat tatttctgat tgcgggcgtt tttgggcggg tttcaatcta 1981 actgtgcccg attttaattc agacaacacg ttagaaagcg atggtgcagg cggtggtaac 2041 atttcagacg gcaaatctac taatggcggc ggtggtggag ctgatgataa atctaccatc 2101 ggtggaggcg caggcggggc tggcggcgga ggcggaggcg gaggtggtgg cggtgatgca 2161 gacggcggtt taggctcaaa tgtctcttta ggcaacacag tcggcacctc aactattgta 2221 ctggtttcgg gcgccgtttt tggtttgacc ggtctgagac gagtgcgatt tttttcgttt 2281 ctaatagctt ccaacaattg ttgtctgtcg tctaaaggtg cagcgggttg aggttccgtc 2341 ggcattggtg gagcgggcgg caattcagac atcgatggtg gtggtggtgg tggaggcgct 2401 ggaatgttag gcacgggaga aggtggtggc ggcggtgccg ccggtataat ttgttctggt 2461 ttagtttgtt cgcgcacgat tgtgggcacc ggcgcaggcg ccgctggctg cacaacggaa 2521 ggtcgtctgc ttcgaggcag cgcttggggt ggtggcaatt caatattata attggaatac 2581 aaatcgtaaa aatctgctat aagcattgta atttcgctat cgtttaccgt gccgatattt 2641 aacaaccgct caatgtaagc aattgtattg taaagagatt gtctcaagct cggatcgatc 2701 ccgcacgccg ataacaagcc ttttcatttt tactacagca ttgtagtggc gagacacttc 2761 gctgtcgtcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 2821 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 2881 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 2941 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 3001 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg 3061 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 3121 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 3181 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 3241 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg 3301 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc 3361 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 3421 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 3481 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt 3541 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 3601 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 3661 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 3721 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 3781 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 3841 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 3901 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 3961 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 4021 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 4081 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 4141 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 4201 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 4261 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 4321 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 4381 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 4441 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 4501 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 4561 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 4621 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 4681 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 4741 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 4801 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 4861 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 4921 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 4981 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 5041 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 5101 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 5161 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 5221 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 5281 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 5341 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 5401 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 5461 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 5521 gccgattcat taatgcag //

   
   
   

2. pBacPAK8 플라스미드 맵

   

   
   
   

pBR322

1. pBR3228 시퀀스

   NG1.0 pBR322 circular dsDNA 4361 bp DESCRIPTION Cloning vector pBR322, complete genome. DATE 2000/12/14 AUTHOR Gilbert,W. FEATURE Source 1..1762 F pSC101 CDS 86..1276 F Tet CDS 1915..2106 F ROP CDS 3293..4153 R Amp Promoter 43..49 F P2 Promoter 4188..4194 R P3 SEQUENCE 1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac 421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca 481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg 541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct 601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat 661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt 721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct 781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct 841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa 901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt 961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc 1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca 1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc 1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat 1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg 1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga 1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac 1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg 1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg 1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc 1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg 1621 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca 1681 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga 1741 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa 1801 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt 1861 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc 1921 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt 1981 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac 2041 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac 2101 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 2161 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc 2221 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg 2281 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc 2341 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 2401 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 2461 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 2521 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 2581 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 2641 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 2701 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 2761 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 2821 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 2881 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 2941 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 3001 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 3061 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 3121 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 3181 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 3241 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 3301 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 3361 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 3421 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 3481 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 3541 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 3601 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 3661 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 3721 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 3781 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 3841 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 3901 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 3961 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 4021 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 4081 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 4141 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 4201 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 4261 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 4321 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga a //

   
   
   

2. pBR3228 플라스미드 맵

   

   
   
   

pcDNA3.1(+)Hygro

1. pcDNA3.1(+)Hygro 시퀀스

   NG1.0 pcDNA3.1(+)Hygro circular dsDNA 5597 bp DESCRIPTION pcDNA3.1(+)Hygro complete sequence from Invitrogen FEATURE CDS 4601..5461 R Amp CDS 2118..3141 F Hygro Promoter 209..863 F pCMV Promoter 1776..2100 F pSV40 Other 895..1010 F MCS Other 1021..1234 F BGH_pA Other 3154..3526 F SV_pA Other 3786..4456 R pUC_Ori SEQUENCE 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc 961 agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca 1021 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 1081 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 1141 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 1201 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 1261 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 1321 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 1381 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 1441 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 1501 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 1561 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 1621 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 1681 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 1741 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 1801 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 1861 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 1921 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 1981 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 2041 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 2101 gatctgatca gcacgtgatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc 2161 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc 2221 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg 2281 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc 2341 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg 2401 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg 2461 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc 2521 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg 2581 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg 2641 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg 2701 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca 2761 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct 2821 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg 2881 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct 2941 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg 3001 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg 3061 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca 3121 ctcgtccgag ggcaaaggaa tagcacgtgc tacgagattt cgattccacc gccgccttct 3181 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 3241 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt 3301 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 3361 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta 3421 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 3481 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 3541 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 3601 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 3661 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 3721 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 3781 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 3841 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 3901 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 3961 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 4021 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 4081 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 4141 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 4201 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 4261 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 4321 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 4381 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 4441 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 4501 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 4561 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 4621 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 4681 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 4741 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 4801 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 4861 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 4921 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 4981 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 5041 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 5101 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 5161 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 5221 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 5281 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 5341 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 5401 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 5461 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 5521 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 5581 aagtgccacc tgacgtc //

   
   
   

2. pcDNA3.1(+)Hygr 플라스미드 맵

   

   
   
   

pcDNA3

1. pcDNA3 시퀀스

   NG1.0 pcDNA3 circular dsDNA 5446 bp DESCRIPTION Cloning vector pcDNA3 from Invitrogen FEATURE CDS 2151..2945 F Neomycin CDS 4450..5310 R Amp Promoter 209..863 F pCMV Promoter 1790..2115 F SV40 Other 1018..1249 F BGH polyA Other 889..994 F MCS Other 3632..4305 F CoE1_Ori SEQUENCE 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atccatcaca 961 ctggcggccg ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta 1021 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 1081 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 1141 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 1201 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 1261 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 1321 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 1381 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 1441 gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac 1501 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 1561 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 1621 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 1681 tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 1741 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca 1801 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 1861 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 1921 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 1981 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc 2041 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt 2101 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac 2161 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 2221 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 2281 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg 2341 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 2401 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 2461 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 2521 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 2581 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 2641 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc 2701 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 2761 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 2821 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 2881 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 2941 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg 3001 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 3061 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa 3121 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 3181 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 3241 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt 3301 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 3361 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 3421 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 3481 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 3541 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 3601 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 3661 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 3721 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 3781 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 3841 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag 3901 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 3961 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 4021 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 4081 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 4141 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 4201 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 4261 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 4321 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 4381 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 4441 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 4501 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 4561 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 4621 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 4681 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 4741 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 4801 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 4861 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 4921 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 4981 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 5041 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 5101 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 5161 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 5221 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 5281 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 5341 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 5401 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc //

   
   
   

2. pcDNA 플라스미드 맵

   

   
   
   

pGEX-2T

1. pGEX-2T 시퀀스

   NG1.0 pGEX-2T circular dsDNA 4948 bp DESCRIPTION pGEX-2T cloning vector, complete sequence. Genbank:U13850 FEATURE CDS 258..956 F Glutathione-S-transferase CDS 1356..2216 F Amp CDS 3297..4379 F LacIq Promoter 183..257 F pTac Promoter 1286..1314 F bla Other 217..237 F lacI_binding Other 2974 F Rep_Origin SEQUENCE 1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccccggg aattcatcgt gactgactga 961 cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 1021 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 1081 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag 1141 tgtataattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 1201 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 1261 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 1321 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 1381 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 1441 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 1501 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 1561 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac 1621 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 1681 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 1741 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 1801 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 1861 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc 1921 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 1981 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 2041 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 2101 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 2161 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 2221 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 2281 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 2341 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 2401 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 2461 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 2521 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 2581 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 2641 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 2701 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 2761 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 2821 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 2881 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 2941 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 3001 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 3061 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 3121 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc 3181 ttacgcatct gtgcggtatt tcacaccgca taaattccga caccatcgaa tggtgcaaaa 3241 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga 3301 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc 3361 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga 3421 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt 3481 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg 3541 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa 3601 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc 3661 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta 3721 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct 3781 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa 3841 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc 3901 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg 3961 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga 4021 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc 4081 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt 4141 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg 4201 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct 4261 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt 4321 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 4381 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg 4441 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc 4501 tatgaccatg attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 4561 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 4621 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg 4681 ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt gcgatcttcc 4741 tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat 4801 ctacaccaac gtaacctatc ccattacggt caatccgccg tttgttccca cggagaatcc 4861 gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg aaggccagac 4921 gcgaattatt tttgatggcg ttggaatt //

   
   
   

2. pGEX-2T 플라스미드 맵

   

   
   
   

pUC19

1. pUC19 시퀀스

   NG1.0 Genbank:X02514 circular dsDNA 2686 bp DESCRIPTION Synthetic construct pUC19c cloning vector sequence. Yanisch-Perron,C., Vieira,J. and Messing,J. (1985) Gene 33 (1), 103-119 (1985) FEATURE CDS 1626..2486 R AmpR CDS 146..469 R LacZa Promoter 455..682 R Lac_promoter Promoter 2521 R AmpR_promoter Other 867..1400 R Ori Other 396..452 R MCS SEQUENCE 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 421 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct 481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 601 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 661 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 721 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 781 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 841 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 901 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 961 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 1021 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 1081 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 1141 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 1201 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 1261 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 1321 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 1381 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 1441 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 1501 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 1561 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 1621 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 1681 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 1741 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 1801 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 1861 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 1921 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 1981 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 2041 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 2101 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 2161 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 2221 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 2281 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 2341 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 2401 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 2461 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 2521 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 2581 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 2641 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc //

   
   
   

2. pUC19 플라스미드 맵

   

   
   
   

GFP

1. GFP 시퀀스

   LOCUS AEVGFPA 966 bp mRNA linear INV 26-APR-1993 DEFINITION Aequorea victoria green-fluorescent protein mRNA, complete cds. ACCESSION M62653 VERSION M62653.1 GI:155660 KEYWORDS aequorin-associated energy-transfer acceptor; bioluminescence; green-fluorescent protein; photocyte. SOURCE Aequorea victoria (library: lambda gt10) cDNA to mRNA. ORGANISM Aequorea victoria Eukaryota; Metazoa; Cnidaria; Hydrozoa; Hydroida; Leptomedusae; Aequoreidae; Aequorea. REFERENCE 1 (bases 1 to 966) AUTHORS Prasher,D.C., Eckenrode,V.K., Ward,W.W., Prendergast,F.G. and Cormier,M.J. TITLE Primary structure of the Aequorea victoria green-fluorescent protein JOURNAL Gene 111 (2), 229-233 (1992) MEDLINE 92175527 PUBMED 1347277 FEATURES Location/Qualifiers source 1..966 /organism="Aequorea victoria" /db_xref="taxon:6100" /tissue_lib="lambda gt10" gene 1..966 /gene="GFP" CDS 26..742 /gene="GFP" /codon_start=1 /product="green-fluorescent protein" /protein_id="AAA27721.1" /db_xref="GI:155661" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITHGMDELYK" misc_feature 218..226 /gene="GFP" /note="fluorescent chromophore" /evidence=experimental BASE COUNT 336 a 169 c 175 g 286 t ORIGIN 1 tacacacgaa taaaagataa caaagatgag taaaggagaa gaacttttca ctggagttgt 61 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga 121 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa 181 actacctgtt ccatggccaa cacttgtcac tactttctct tatggtgttc aatgcttttc 241 aagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta 301 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt 361 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga 421 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat 481 catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga 541 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc 601 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa 661 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg 721 catggatgaa ctatacaaat aaatgtccag acttccaatt gacactaaag tgtccgaaca 781 attactaaaa tctcagggtt cctggttaaa ttcaggctga gatattattt atatatttat 841 agattcatta aaattgtatg aataatttat tgatgttatt gatagaggtt attttcttat 901 taaacaggct acttggagtg tattcttaat tctatattaa ttacaatttg atttgacttg 961 ctcaaa //

   
   
   

2. GFP 플라스미드 맵