Full List of all Restriction EnzymesTop

Enzyme	Recognition Seq	Palindrome?
AarI	CACCTGCnnnn/nnnn_	No
AatII	GACGT/C	Yes
AbsI	CC/TCGAGG	Yes
Acc65I	G/GTACC	Yes
AccI	GT/mkAC	Yes
AciI	C/CG_C	No
AclI	AA/CGTT	Yes
AcuI	CTGAAGnnnnnnnnnnnnnn_nn/	No
AfeI	AGC/GCT	Yes
AflII	C/TTAAG	Yes
AflIII	A/CryGT	Yes
AgeI	A/CCGGT	Yes
AhdI	GACnnn/nnGTC	Yes
AjuI	CCAAnnnnnnnTTCnnnnnnn_nnnnn/	No
AleI	CACnn/nnGTG	Yes
AlfI	GCAnnnnnnTGCnnnnnnnnnn_nn/	No
AloI	GGAnnnnnnGTTCnnnnnnn_nnnnn/	No
AluI	AG/CT	Yes
AlwI	GGATCnnnn/n_	No
AlwNI	CAGnnn/CTG	Yes
ApaI	GGGCC/C	Yes
ApaLI	G/TGCAC	Yes
ApoI	r/AATTy	Yes
AscI	GG/CGCGCC	Yes
AseI	AT/TAAT	Yes
AsiSI	GCGAT/CGC	Yes
AvaI	C/yCGrG	Yes
AvaII	G/GwCC	Yes
AvrII	C/CTAGG	Yes
BaeI	GrTACnnnnGTnnnnnnnnnn_nnnnn/	No
BamHI	G/GATCC	Yes
BanI	G/GyrCC	Yes
BanII	GrGCy/C	Yes
BarI	GTAnnnnnnCTTCnnnnnnn_nnnnn/	No
BbeI	GGCGC/C	Yes
BbsI	GAAGACnn/nnnn_	No
BbvCI	CC/TCA_GC	No
BbvI	GCAGCnnnnnnnn/nnnn_	No
BccI	CCATCnnnn/n_	No
BceAI	ACGGCnnnnnnnnnnnn/nn_	No
BcgI	GCAnnnnnnTCGnnnnnnnnnn_nn/	No
BciVI	GTATCCnnnnn_n/	No
BclI	T/GATCA	Yes
BdaI	TGAnnnnnnTCAnnnnnnnnnn_nn/	No
BfaI	C/TAG	Yes
BglI	GCCnnnn/nGGC	Yes
BglII	A/GATCT	Yes
BisI	GC/nGC	Yes
BlpI	GC/TnAGC	Yes
BlsI	GC/nG_C	No
Bme1580I	GkGCm/C	Yes
BmeT110I	Cy/CGrG	Yes
BmgBI	CAC/_GTC	No
BmgT120I	GG/nCC	Yes
BmrI	ACTGGGnnnn_n/	No
BmtI	GCTAG/C	Yes
BplI	GAGnnnnnCTCnnnnnnnn_nnnnn/	No
BpmI	CTGGAGnnnnnnnnnnnnnn_nn/	No
Bpu10I	CC/TnA_GC	No
BpuEI	CTTGAGnnnnnnnnnnnnnn_nn/	No
BsaAI	yAC/GTr	Yes
BsaBI	GATnn/nnATC	Yes
BsaHI	Gr/CGyC	Yes
BsaI	GGTCTCn/nnnn_	No
BsaJI	C/CnnGG	Yes
BsaWI	w/CCGGw	Yes
BsaXI	GGAGnnnnnGTnnnnnnnnn_nnn/	No
BseMII	CTCAGnnnnnnnn_nn/	No
BseRI	GAGGAGnnnnnnnn_nn/	No
BseYI	C/CCAG_C	No
BsgI	GTGCAGnnnnnnnnnnnnnn_nn/	No
BsiEI	CGry/CG	Yes
BsiHKAI	GwGCw/C	Yes
BsiWI	C/GTACG	Yes
BslI	CCnnnnn/nnGG	Yes
BsmAI	GTCTCn/nnnn_	No
BsmBI	CGTCTCn/nnnn_	No
BsmFI	GGGACnnnnnnnnnn/nnnn_	No
BsmI	GAATG_Cn/	No
Bsp1286I	GdGCh/C	Yes
BspCNI	CTCAGnnnnnnn_nn/	No
BspEI	T/CCGGA	Yes
BspHI	T/CATGA	Yes
BspMI	ACCTGCnnnn/nnnn_	No
BsrBI	CCG/_CTC	No
BsrDI	GCAATG_nn/	No
BsrFI	r/CCGGy	Yes
BsrGI	T/GTACA	Yes
BsrI	ACTG_Gn/	No
BssHII	G/CGCGC	Yes
BssSI	C/ACGA_G	No
BstAPI	GCAnnnn/nTGC	Yes
BstBI	TT/CGAA	Yes
BstEII	G/GTnACC	Yes
BstKTI	GAT/C	Yes
BstNI	CC/wGG	Yes
BstUI	CG/CG	Yes
BstXI	CCAnnnnn/nTGG	Yes
BstYI	r/GATCy	Yes
BstZ17I	GTA/TAC	Yes
Bsu36I	CC/TnAGG	Yes
BtgI	C/CryGG	Yes
BtgZI	GCGATGnnnnnnnnnn/nnnn_	No
BtsCI	GGATG_nn/	No
BtsI	GCAGTG_nn/	No
Cac8I	GCn/nGC	Yes
ClaI	AT/CGAT	Yes
Cre	ATAACTTCGTATAG_CATACA/TTATACGAAGTTAT	No
CspCI	CCACnnnnnTTGnnnnnnnnnnn_nn/	No
CviAII	C/ATG	Yes
CviKI-1	rG/Cy	Yes
CviQI	G/TAC	Yes
DdeI	C/TnAG	Yes
DpnI	GA/TC	Yes
DraI	TTT/AAA	Yes
DraIII	CACnnn/GTG	Yes
DrdI	GACnnnn/nnGTC	Yes
EaeI	y/GGCCr	Yes
EagI	C/GGCCG	Yes
EarI	CTCTTCn/nnn_	No
EciI	GGCGGAnnnnnnnnn_nn/	No
Eco57MI	CTGrAGnnnnnnnnnnnnnn_nn/	No
EcoICRI	GAG/CTC	Yes
EcoNI	CCTnn/nnnAGG	Yes
EcoO109I	rG/GnCCy	Yes
EcoRI	G/AATTC	Yes
EcoRV	GAT/ATC	Yes
FalI	AAGnnnnnCTTnnnnnnnn_nnnnn/	No
FatI	/CATG	Yes
FauI	CCCGCnnnn/nn_	No
Fnu4HI	GC/nGC	Yes
FokI	GGATGnnnnnnnnn/nnnn_	No
FseI	GGCCGG/CC	Yes
FspAI	rTGC/GCAy	Yes
FspI	TGC/GCA	Yes
GlaI	GC/GC	Yes
HaeII	rGCGC/y	Yes
HaeIII	GG/CC	Yes
HgaI	GACGCnnnnn/nnnnn_	No
HhaI	GCG/C	Yes
Hin4I	GAbnnnnnrTCnnnnnnnn_nnnnn/	No
HinP1I	G/CGC	Yes
HincII	GTy/rAC	Yes
HindIII	A/AGCTT	Yes
HinfI	G/AnTC	Yes
HpaI	GTT/AAC	Yes
HpaII	C/CGG	Yes
HphI	GGTGAnnnnnnn_n/	No
Hpy188I	TCn/GA	Yes
Hpy188III	TC/nnGA	Yes
Hpy8I	GTn/nAC	Yes
Hpy99I	CGwCG/	Yes
HpyAV	CCTTCnnnnn_n/	No
HpyCH4III	ACn/GT	Yes
HpyCH4IV	A/CGT	Yes
HpyCH4V	TG/CA	Yes
KasI	G/GCGCC	Yes
KpnI	GGTAC/C	Yes
MaeIII	/GTnAC	Yes
MboI	/GATC	Yes
MboII	GAAGAnnnnnnn_n/	No
MfeI	C/AATTG	Yes
MluI	A/CGCGT	Yes
MlyI	GAGTCnnnnn/_	No
MmeI	TCCrACnnnnnnnnnnnnnnnnnn_nn/	No
MnlI	CCTCnnnnnn_n/	No
MreI	CG/CCGGCG	Yes
MscI	TGG/CCA	Yes
MseI	T/TAA	Yes
MslI	CAynn/nnrTG	Yes
MspA1I	CmG/CkG	Yes
MwoI	GCnnnnn/nnGC	Yes
NaeI	GCC/GGC	Yes
NarI	GG/CGCC	Yes
NciI	CC/sGG	Yes
NcoI	C/CATGG	Yes
NdeI	CA/TATG	Yes
NgoMIV	G/CCGGC	Yes
NheI	G/CTAGC	Yes
NlaIII	CATG/	Yes
NlaIV	GGn/nCC	Yes
NmeAIII	GCCGAGnnnnnnnnnnnnnnnnnnn_nn/	No
NotI	GC/GGCCGC	Yes
NruI	TCG/CGA	Yes
NsiI	ATGCA/T	Yes
NspI	rCATG/y	Yes
PacI	TTAAT/TAA	Yes
PasI	CC/CwGGG	Yes
PciI	A/CATGT	Yes
PflMI	CCAnnnn/nTGG	Yes
PfoI	T/CCnGGA	Yes
PleI	GAGTCnnnn/n_	No
PmeI	GTTT/AAAC	Yes
PmlI	CAC/GTG	Yes
PpiI	GAGnnnnnGTTCnnnnnnn_nnnnn/	No
PpuMI	rG/GwCCy	Yes
PshAI	GACnn/nnGTC	Yes
PsiI	TTA/TAA	Yes
PspGI	/CCwGG	Yes
PspOMI	G/GGCCC	Yes
PspXI	vC/TCGAGb	Yes
PsrI	GTAnnnnnnGTTCnnnnnnn_nnnnn/	No
PstI	CTGCA/G	Yes
PvuI	CGAT/CG	Yes
PvuII	CAG/CTG	Yes
RsaI	GT/AC	Yes
RsrII	CG/GwCCG	Yes
SacI	GAGCT/C	Yes
SacII	CCGC/GG	Yes
SalI	G/TCGAC	Yes
SanDI	GG/GwCCC	Yes
SapI	GCTCTTCn/nnn_	No
Sau96I	G/GnCC	Yes
SbfI	CCTGCA/GG	Yes
ScaI	AGT/ACT	Yes
ScrFI	CC/nGG	Yes
SetI	AssT/	Yes
SexAI	A/CCwGGT	Yes
SfaNI	GCATCnnnnn/nnnn_	No
SfcI	C/TryAG	Yes
SfiI	GGCCnnnn/nGGCC	Yes
SfoI	GGC/GCC	Yes
SgrAI	Cr/CCGGyG	Yes
SgrDI	CG/TCGACG	Yes
SmaI	CCC/GGG	Yes
SmlI	C/TyrAG	Yes
SnaBI	TAC/GTA	Yes
SpeI	A/CTAGT	Yes
SphI	GCATG/C	Yes
SrfI	GCCC/GGGC	Yes
SspI	AAT/ATT	Yes
StuI	AGG/CCT	Yes
StyD4I	/CCnGG	Yes
StyI	C/CwwGG	Yes
SwaI	ATTT/AAAT	Yes
TaiI	ACGT/	Yes
TaqI	T/CGA	Yes
TaqII	CACCCAnnnnnnnnn_nn/	No
TatI	w/GTACw	Yes
TauI	GCsG/C	Yes
TfiI	G/AwTC	Yes
TseI	G/CwGC	Yes
TsoI	TArCCAnnnnnnnnn_nn/	No
Tsp45I	/GTsAC	Yes
Tsp509I	/AATT	Yes
TspDTI	ATGAAnnnnnnnnn_nn/	No
TspGWI	ACGGAnnnnnnnnn_nn/	No
TspRI	nnCAsTGnn/	Yes
TstI	GGAnnnnnnGTGnnnnnnnn_nnnnn/	No
Tth111I	GACn/nnGTC	Yes
XbaI	T/CTAGA	Yes
XcmI	CCAnnnnn/nnnnTGG	Yes
XhoI	C/TCGAG	Yes
XmaI	C/CCGGG	Yes
XmnI	GAAnn/nnTTC	Yes
ZraI	GAC/GTC	Yes
Total 256 Enzymes

Popular EnzymesTop

Enzyme	Recognition Seq	Palindrome?
ApaI	GGGCC/C	Yes
BamHI	G/GATCC	Yes
BglII	A/GATCT	Yes
ClaI	AT/CGAT	Yes
EcoRI	G/AATTC	Yes
HindIII	A/AGCTT	Yes
HpaI	GTT/AAC	Yes
KpnI	GGTAC/C	Yes
MluI	A/CGCGT	Yes
NcoI	C/CATGG	Yes
NdeI	CA/TATG	Yes
NheI	G/CTAGC	Yes
NotI	GC/GGCCGC	Yes
PacI	TTAAT/TAA	Yes
PstI	CTGCA/G	Yes
PvuII	CAG/CTG	Yes
SacI	GAGCT/C	Yes
SalI	G/TCGAC	Yes
SfiI	GGCCnnnn/nGGCC	Yes
SpeI	A/CTAGT	Yes
XbaI	T/CTAGA	Yes
XhoI	C/TCGAG	Yes
Total 22 Enzymes

General EnzymesTop

Enzyme	Recognition Seq	Palindrome?
AatII	GACGT/C	Yes
Acc65I	G/GTACC	Yes
AclI	AA/CGTT	Yes
AfeI	AGC/GCT	Yes
AflII	C/TTAAG	Yes
AgeI	A/CCGGT	Yes
AhdI	GACnnn/nnGTC	Yes
AleI	CACnn/nnGTG	Yes
AlwNI	CAGnnn/CTG	Yes
ApaI	GGGCC/C	Yes
ApaLI	G/TGCAC	Yes
AscI	GG/CGCGCC	Yes
AseI	AT/TAAT	Yes
AsiSI	GCGAT/CGC	Yes
AvrII	C/CTAGG	Yes
BamHI	G/GATCC	Yes
BbeI	GGCGC/C	Yes
BclI	T/GATCA	Yes
BglI	GCCnnnn/nGGC	Yes
BglII	A/GATCT	Yes
BlpI	GC/TnAGC	Yes
BsaBI	GATnn/nnATC	Yes
BsiWI	C/GTACG	Yes
BspEI	T/CCGGA	Yes
BspHI	T/CATGA	Yes
BsrGI	T/GTACA	Yes
BssHII	G/CGCGC	Yes
BstAPI	GCAnnnn/nTGC	Yes
BstBI	TT/CGAA	Yes
BstEII	G/GTnACC	Yes
BstXI	CCAnnnnn/nTGG	Yes
BstZ17I	GTA/TAC	Yes
Bsu36I	CC/TnAGG	Yes
ClaI	AT/CGAT	Yes
DraI	TTT/AAA	Yes
DraIII	CACnnn/GTG	Yes
DrdI	GACnnnn/nnGTC	Yes
EagI	C/GGCCG	Yes
EcoICRI	GAG/CTC	Yes
EcoNI	CCTnn/nnnAGG	Yes
EcoRI	G/AATTC	Yes
EcoRV	GAT/ATC	Yes
FseI	GGCCGG/CC	Yes
FspI	TGC/GCA	Yes
HindIII	A/AGCTT	Yes
HpaI	GTT/AAC	Yes
KasI	G/GCGCC	Yes
KpnI	GGTAC/C	Yes
MfeI	C/AATTG	Yes
MluI	A/CGCGT	Yes
MscI	TGG/CCA	Yes
NaeI	GCC/GGC	Yes
NarI	GG/CGCC	Yes
NcoI	C/CATGG	Yes
NdeI	CA/TATG	Yes
NgoMIV	G/CCGGC	Yes
NheI	G/CTAGC	Yes
NotI	GC/GGCCGC	Yes
NruI	TCG/CGA	Yes
NsiI	ATGCA/T	Yes
PacI	TTAAT/TAA	Yes
PciI	A/CATGT	Yes
PflMI	CCAnnnn/nTGG	Yes
PmeI	GTTT/AAAC	Yes
PmlI	CAC/GTG	Yes
PshAI	GACnn/nnGTC	Yes
PsiI	TTA/TAA	Yes
PspOMI	G/GGCCC	Yes
PstI	CTGCA/G	Yes
PvuI	CGAT/CG	Yes
PvuII	CAG/CTG	Yes
RsrII	CG/GwCCG	Yes
SacI	GAGCT/C	Yes
SacII	CCGC/GG	Yes
SalI	G/TCGAC	Yes
SanDI	GG/GwCCC	Yes
SbfI	CCTGCA/GG	Yes
ScaI	AGT/ACT	Yes
SexAI	A/CCwGGT	Yes
SfiI	GGCCnnnn/nGGCC	Yes
SfoI	GGC/GCC	Yes
SgrAI	Cr/CCGGyG	Yes
SmaI	CCC/GGG	Yes
SnaBI	TAC/GTA	Yes
SpeI	A/CTAGT	Yes
SphI	GCATG/C	Yes
SrfI	GCCC/GGGC	Yes
SspI	AAT/ATT	Yes
StuI	AGG/CCT	Yes
SwaI	ATTT/AAAT	Yes
Tth111I	GACn/nnGTC	Yes
XbaI	T/CTAGA	Yes
XcmI	CCAnnnnn/nnnnTGG	Yes
XhoI	C/TCGAG	Yes
XmaI	C/CCGGG	Yes
XmnI	GAAnn/nnTTC	Yes
Total 96 Enzymes

Default EnzymesTop

Enzyme	Recognition Seq	Palindrome?
ApaI	GGGCC/C	Yes
AscI	GG/CGCGCC	Yes
BamHI	G/GATCC	Yes
BclI	T/GATCA	Yes
BglII	A/GATCT	Yes
Bsu36I	CC/TnAGG	Yes
ClaI	AT/CGAT	Yes
EagI	C/GGCCG	Yes
EcoRI	G/AATTC	Yes
EcoRV	GAT/ATC	Yes
HindIII	A/AGCTT	Yes
HpaI	GTT/AAC	Yes
KpnI	GGTAC/C	Yes
MluI	A/CGCGT	Yes
NcoI	C/CATGG	Yes
NdeI	CA/TATG	Yes
NheI	G/CTAGC	Yes
NotI	GC/GGCCGC	Yes
NruI	TCG/CGA	Yes
PacI	TTAAT/TAA	Yes
PmeI	GTTT/AAAC	Yes
PstI	CTGCA/G	Yes
PvuII	CAG/CTG	Yes
SacI	GAGCT/C	Yes
SalI	G/TCGAC	Yes
ScaI	AGT/ACT	Yes
SexAI	A/CCwGGT	Yes
SfiI	GGCCnnnn/nGGCC	Yes
SmaI	CCC/GGG	Yes
SpeI	A/CTAGT	Yes
SwaI	ATTT/AAAT	Yes
XbaI	T/CTAGA	Yes
XhoI	C/TCGAG	Yes
XmaI	C/CCGGG	Yes
Total 34 Enzymes

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